Lignin content in natural Populus variants affects sugar release.
Identifieur interne : 002E05 ( Main/Exploration ); précédent : 002E04; suivant : 002E06Lignin content in natural Populus variants affects sugar release.
Auteurs : Michael H. Studer [États-Unis] ; Jaclyn D. Demartini ; Mark F. Davis ; Robert W. Sykes ; Brian Davison ; Martin Keller ; Gerald A. Tuskan ; Charles E. WymanSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2011.
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Abstract
The primary obstacle to producing renewable fuels from lignocellulosic biomass is a plant's recalcitrance to releasing sugars bound in the cell wall. From a sample set of wood cores representing 1,100 individual undomesticated Populus trichocarpa trees, 47 extreme phenotypes were selected across measured lignin content and ratio of syringyl and guaiacyl units (S/G ratio). This subset was tested for total sugar release through enzymatic hydrolysis alone as well as through combined hot-water pretreatment and enzymatic hydrolysis using a high-throughput screening method. The total amount of glucan and xylan released varied widely among samples, with total sugar yields of up to 92% of the theoretical maximum. A strong negative correlation between sugar release and lignin content was only found for pretreated samples with an S/G ratio < 2.0. For higher S/G ratios, sugar release was generally higher, and the negative influence of lignin was less pronounced. When examined separately, only glucose release was correlated with lignin content and S/G ratio in this manner, whereas xylose release depended on the S/G ratio alone. For enzymatic hydrolysis without pretreatment, sugar release increased significantly with decreasing lignin content below 20%, irrespective of the S/G ratio. Furthermore, certain samples featuring average lignin content and S/G ratios exhibited exceptional sugar release. These facts suggest that factors beyond lignin and S/G ratio influence recalcitrance to sugar release and point to a critical need for deeper understanding of cell-wall structure before plants can be rationally engineered for reduced recalcitrance and efficient biofuels production.
DOI: 10.1073/pnas.1009252108
PubMed: 21444820
PubMed Central: PMC3076829
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Wyman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21444820</idno><idno type="pmid">21444820</idno><idno type="doi">10.1073/pnas.1009252108</idno><idno type="pmc">PMC3076829</idno><idno type="wicri:Area/Main/Corpus">002E60</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002E60</idno><idno type="wicri:Area/Main/Curation">002E60</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002E60</idno><idno type="wicri:Area/Main/Exploration">002E60</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Lignin content in natural Populus variants affects sugar release.</title><author><name sortKey="Studer, Michael H" sort="Studer, Michael H" uniqKey="Studer M" first="Michael H" last="Studer">Michael H. Studer</name><affiliation wicri:level="2"><nlm:affiliation>University of California, Bourns College of Engineering, Center for Environmental Research and Technology, Riverside, CA 92507, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of California, Bourns College of Engineering, Center for Environmental Research and Technology, Riverside, CA 92507</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Demartini, Jaclyn D" sort="Demartini, Jaclyn D" uniqKey="Demartini J" first="Jaclyn D" last="Demartini">Jaclyn D. Demartini</name></author><author><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name></author><author><name sortKey="Sykes, Robert W" sort="Sykes, Robert W" uniqKey="Sykes R" first="Robert W" last="Sykes">Robert W. Sykes</name></author><author><name sortKey="Davison, Brian" sort="Davison, Brian" uniqKey="Davison B" first="Brian" last="Davison">Brian Davison</name></author><author><name sortKey="Keller, Martin" sort="Keller, Martin" uniqKey="Keller M" first="Martin" last="Keller">Martin Keller</name></author><author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbohydrate Metabolism (MeSH)</term><term>Glucans (metabolism)</term><term>Lignin (analysis)</term><term>Populus (chemistry)</term><term>Populus (metabolism)</term><term>Xylans (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Glucanes (métabolisme)</term><term>Lignine (analyse)</term><term>Métabolisme glucidique (MeSH)</term><term>Populus (composition chimique)</term><term>Populus (métabolisme)</term><term>Xylanes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucans</term><term>Xylans</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucanes</term><term>Populus</term><term>Xylanes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Metabolism</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Métabolisme glucidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The primary obstacle to producing renewable fuels from lignocellulosic biomass is a plant's recalcitrance to releasing sugars bound in the cell wall. From a sample set of wood cores representing 1,100 individual undomesticated Populus trichocarpa trees, 47 extreme phenotypes were selected across measured lignin content and ratio of syringyl and guaiacyl units (S/G ratio). This subset was tested for total sugar release through enzymatic hydrolysis alone as well as through combined hot-water pretreatment and enzymatic hydrolysis using a high-throughput screening method. The total amount of glucan and xylan released varied widely among samples, with total sugar yields of up to 92% of the theoretical maximum. A strong negative correlation between sugar release and lignin content was only found for pretreated samples with an S/G ratio < 2.0. For higher S/G ratios, sugar release was generally higher, and the negative influence of lignin was less pronounced. When examined separately, only glucose release was correlated with lignin content and S/G ratio in this manner, whereas xylose release depended on the S/G ratio alone. For enzymatic hydrolysis without pretreatment, sugar release increased significantly with decreasing lignin content below 20%, irrespective of the S/G ratio. Furthermore, certain samples featuring average lignin content and S/G ratios exhibited exceptional sugar release. These facts suggest that factors beyond lignin and S/G ratio influence recalcitrance to sugar release and point to a critical need for deeper understanding of cell-wall structure before plants can be rationally engineered for reduced recalcitrance and efficient biofuels production.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21444820</PMID><DateCompleted><Year>2011</Year><Month>06</Month><Day>27</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>108</Volume><Issue>15</Issue><PubDate><Year>2011</Year><Month>Apr</Month><Day>12</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation></Journal><ArticleTitle>Lignin content in natural Populus variants affects sugar release.</ArticleTitle><Pagination><MedlinePgn>6300-5</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.1009252108</ELocationID><Abstract><AbstractText>The primary obstacle to producing renewable fuels from lignocellulosic biomass is a plant's recalcitrance to releasing sugars bound in the cell wall. From a sample set of wood cores representing 1,100 individual undomesticated Populus trichocarpa trees, 47 extreme phenotypes were selected across measured lignin content and ratio of syringyl and guaiacyl units (S/G ratio). This subset was tested for total sugar release through enzymatic hydrolysis alone as well as through combined hot-water pretreatment and enzymatic hydrolysis using a high-throughput screening method. The total amount of glucan and xylan released varied widely among samples, with total sugar yields of up to 92% of the theoretical maximum. A strong negative correlation between sugar release and lignin content was only found for pretreated samples with an S/G ratio < 2.0. For higher S/G ratios, sugar release was generally higher, and the negative influence of lignin was less pronounced. When examined separately, only glucose release was correlated with lignin content and S/G ratio in this manner, whereas xylose release depended on the S/G ratio alone. For enzymatic hydrolysis without pretreatment, sugar release increased significantly with decreasing lignin content below 20%, irrespective of the S/G ratio. Furthermore, certain samples featuring average lignin content and S/G ratios exhibited exceptional sugar release. These facts suggest that factors beyond lignin and S/G ratio influence recalcitrance to sugar release and point to a critical need for deeper understanding of cell-wall structure before plants can be rationally engineered for reduced recalcitrance and efficient biofuels production.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Studer</LastName><ForeName>Michael H</ForeName><Initials>MH</Initials><AffiliationInfo><Affiliation>University of California, Bourns College of Engineering, Center for Environmental Research and Technology, Riverside, CA 92507, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>DeMartini</LastName><ForeName>Jaclyn D</ForeName><Initials>JD</Initials></Author><Author ValidYN="Y"><LastName>Davis</LastName><ForeName>Mark F</ForeName><Initials>MF</Initials></Author><Author ValidYN="Y"><LastName>Sykes</LastName><ForeName>Robert W</ForeName><Initials>RW</Initials></Author><Author ValidYN="Y"><LastName>Davison</LastName><ForeName>Brian</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Keller</LastName><ForeName>Martin</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Tuskan</LastName><ForeName>Gerald A</ForeName><Initials>GA</Initials></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>03</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S 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IdType="pubmed">17816186</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><name sortKey="Davison, Brian" sort="Davison, Brian" uniqKey="Davison B" first="Brian" last="Davison">Brian Davison</name><name sortKey="Demartini, Jaclyn D" sort="Demartini, Jaclyn D" uniqKey="Demartini J" first="Jaclyn D" last="Demartini">Jaclyn D. Demartini</name><name sortKey="Keller, Martin" sort="Keller, Martin" uniqKey="Keller M" first="Martin" last="Keller">Martin Keller</name><name sortKey="Sykes, Robert W" sort="Sykes, Robert W" uniqKey="Sykes R" first="Robert W" last="Sykes">Robert W. Sykes</name><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Studer, Michael H" sort="Studer, Michael H" uniqKey="Studer M" first="Michael H" last="Studer">Michael H. 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